As the Coronavirus pandemic continues to spread, it is important to keep ourselves and those around us safe and healthy. That is why we stock up on hand sanitisers, face masks, gloves and disinfectants to prevent the spread of COVID-19. But as the virus continues to spread as time goes by, we are being reminded that not all products on the market are created equal. For instance, not all hand sanitisers are as effective as killing pathogens from your hands.

One misconception is that a higher percentage of alcohol concentration in disinfectants provides greater effectiveness in killing away germs and pathogens from the surfaces used.


What are the differences between 70% and 99% Isopropyl alcohol?

99% Isopropyl alcohol is a pure isopropanol whereas 70% isopropyl alcohol is pure isopropanol diluted with 30 percent purified water by volume (CDC, 2020). 70% isopropyl alcohol kills organisms by denaturing their proteins and dissolving their lipids and is effective against most bacteria, fungi and many viruses, but is ineffective against bacterial spores (CDC, 2020).


70% isopropyl alcohol is by far better at killing bacteria and viruses than 99% isopropyl alcohol.

As a disinfectant, 70% concentration of alcohol is the most effective at killing pathogens. Any higher or lower percentage will be less effective. The Centers for Disease Control and Prevention (CDC) did research on disinfecting and sanitizing methods and published the Guideline for Disinfection and Sterilization in Healthcare Facilities.

Use of the more concentrated solutions (99%) will result in almost immediate coagulation of surface or cell wall proteins and prevent passage of the alcohol into the cell. When the outer membrane is coagulated, it protects the virus or bacteria from letting through the isopropyl (Widmer and Frei, 2011). Thus the stronger solution of isopropyl is creating a protection for the germ from the antiseptic properties of isopropyl, rendering the virus or bacteria more resilient against the isopropyl alcohol. To put it simply, higher concentrations cause an external injury that forms a protective wall and shields the organism. Furthermore, 99% isopropanol evaporates very quickly which does not allow it to penetrate cell walls and kill bacteria, and therefore isn’t as good for disinfecting surfaces. In other words, it breaks down the outside of the cell before it can penetrate the pathogen.

In comparison, a 70% isopropyl alcohol can effectiveness cross over the cell membrane, thereby attacking the entire cell and killing the bacteria (CDC, 2020). The benefits of using 70% alcohol are

  1. Coagulation of surface proteins proceeds at a slower pace, thereby allowing the alcohol to enter the cell.
  2. 70% alcohol, being a dilution of absolute alcohol, contains water which is essential in the denaturing process of proteins.
  3. Due to the concentration difference of water and alcohol on either side of the cell wall, 70% alcohol enters the cell to denature both enzymatic and structural proteins. This increases the potency of its antimicrobial properties.

The CDC recommends 70% for disinfecting one’s household and routine cleaning (CDC, 2020)


Important Reminder:
Alcohol percentage and its effectiveness at killing viruses are dependent on the virus in question. Viruses with an envelope structure (Fenner et al., 1987) such as the cold, flu or coronavirus can be broken down by alcohol solutions of 60 percent or higher. However, other viral structures like the norovirus cannot be killed with any concentration of alcohol (Sattar, 2004). When using alcohol to disinfect or sanitize an area, it is advised that the overall germ-killing properties will be enhanced if the area is cleaned first. The cleaning step will help to physically remove any physical soil (dirt, grim), thereby minimizing the amount of soil and protein material that can reduce the potency of the alcohol (CDC, 2020).



Reference list:

Centers for Disease Control and Prevention. 2020. Coronavirus Disease 2019 (COVID-19) – Environmental Cleaning And Disinfection Recommendations. [online] Available at: <> [Accessed 24 August 2020].

Centers for Disease Control and Prevention. 2020. Chemical Disinfectants | Disinfection & Sterilization Guidelines | Guidelines Library | Infection Control | CDC. [online] Available at: <> [Accessed 24 August 2020].

Fenner, F., Bachmann, P., Gibbs, E., Murphy, F., Studdert, M. and White, D., 1987. Structure and Composition of Viruses. Veterinary Virology, pp.3-19.

Sattar, S.A. 2004.  Microbicides and the environmental control of nosocomial viral infections. J. Hosp. Infect. 56, 64–69.

Widmer A, Frei R. 2011. Decontamination, Disinfection, and Sterilization, p 143-173. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch11